A commensal gone bad : complete genome sequence of the prototypical 1 enterotoxigenic Escherichia coli strain H 10407 2 3
نویسندگان
چکیده
The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge, 10 United Kingdom; Department of Veterinary Medicine, University of Cambridge, 11 Cambridge, United Kingdom; School of Chemistry and Molecular Biosciences, 12 University of Queensland, Brisbane, Australia; School of Immunity and Infection and 13 School of Biosciences, University of Birmingham, Birmingham, United Kingdom; 14 Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, 15 Dublin, Ireland; Department of Enteric Infections, Division of Communicable 16 Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, 17 Maryland, USA; School of Biosciences, The University of Nottingham, Sutton 18 Bonington, United Kingdom; Department of Veterinary and Biomedical Sciences, 19 University of Minnesota, Saint Paul, Minnesota. 20
منابع مشابه
A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407,...
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With the plasmid DNA from a clinical isolate of enterotoxigenic Escherichia coli (ETEC) H 10407 as template, PCR-mediated cloning of the sequence encoding the heat-labile toxin B subunit (L T -B) has been carried out. Then this sequence was recloned into the pTrc 99A and pET23a expression vectors to give the pJasmids pTRCLTB and pETLTB, respectively. After induction, the former plasmid provides...
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تاریخ انتشار 2010